The present study was designed to investigate the cytotoxic activity of methanolic extract of Grewia asiatica fruit. In-vitro cytotoxic activity was determined by using MTT assay using cell lines HEK-293 (Epidermal Kidney Cancer cell line), NCI-H522 (Cell Lung cancer cell line), HELA (Cervical Cancer cell line), Hep – 2 (Laryngeal Cancer cell line), and MCF-7 (Breast cancer cell line). To determine its activity, various concentrations (0.005-100 μM/ml) of standard Methotraxate and methanolic extract of G.asiatica fruit extract (dissolved in 2 % DMSO (dimethylsulphoxide) solution) were used. By using ELISA reader at wavelength in between 550 and 600 nm and % cell inhibition was determined. Plotting a graph of Log (concentration of compound) vs % cell inhibition, IC50, the concentration of compound required to inhibit 50 % cell growth, was determined. Comparable cytotoxicity of Methanolic extract of G. asiatica was found against lung cancer cell line and breast cancer cell line respectively but no activity found against normal cell line, Cervical cancer cell line and Larynx cancer cell line. Extract shows in-vitro cytotoxic activity but active constitute remain to be investigation.
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